Pig sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit

Mfr.No.

ELK7917

Sensitivity

0.32 ng/mL

Detection Range

0.79-50 ng/mL

Reactivity

Pig

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

Recovery

Matrices listed below were spiked with certain level of recombinant sC5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of sC5b-9 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	77-95%	86%
Heparin plasma(n=5)	84-97%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sC5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	88-96%	87-98%	95-102%
EDTA plasma(n=5)	85-101%	84-98%	85-93%	86-98%
Heparin plasma(n=5)	86-93%	91-105%	87-98%	89-94%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
50.00	2.043	1.971
25.00	1.643	1.571
12.50	1.186	1.114
6.25	0.752	0.680
3.13	0.527	0.455
1.57	0.321	0.249
0.79	0.206	0.134
0.00	0.072	0.000

Limit Of Detection

50 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207753	ELK7917	48T	$412		Add to Cart
BHE15207753	ELK7917	96T	$588		Add to Cart

Pig sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit

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