Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

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Mfr.No.

CSB-E13178p

Alternative Names

chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Reactivity

Pig

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-94
EDTA plasma (n=4)	94	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-101
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	87-97
1:8	Average %	99
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2200	2.489	2.413	2.451	2.332
1100	2.117	2.098	2.108	1.989
550	1.497	1.452	1.475	1.356
275	0.948	0.939	0.944	0.825
137.5	0.622	0.611	0.617	0.498
68.75	0.375	0.368	0.372	0.253
34.4	0.288	0.281	0.285	0.166
0	0.121	0.117	0.119

Species

Sus scrofa

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	P42831
Antigen	chemokine (C-C motif) ligand 2

Applications

ELISA

Research Area

CCL2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507172	CSB-E13178p	96T	$790	Add to Cart
BHE10507172	CSB-E13178p	5x96T	$3792	Add to Cart
BHE10507172	CSB-E13178p	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.