Pig major acute phase protein (MAP) ELISA Kit

Pig major acute phase protein (MAP) ELISA Kit

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Mfr.No.

CSB-E13425p

Alternative Names

inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein), DKFZp686G21125, H4P, IHRP, ITIHL1, PK120, inter-alpha (globulin) inhibitor H4, inter-alpha-trypsin inhibitor, heavy chain-like, 1, inter-alpha-trypsin inhibitor, heavy chain-related protein, plasma kal,Major acute phase protein(

Reactivity

Pig

Detection Range

37.5 ng/mL-2400 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse CXCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-101
EDTA plasma (n=4)	94	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-102
1:2	Average %	89
	Range %	81-98
1:4	Average %	95
	Range %	85-103
1:8	Average %	96
	Range %	90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.813	2.764	2.789	2.641
250	2.010	1.988	1.999	1.851
125	1.254	1.291	1.273	1.125
62.5	0.747	0.782	0.765	0.617
31.2	0.591	0.582	0.587	0.439
15.6	0.326	0.317	0.322	0.174
7.8	0.215	0.228	0.222	0.074
0	0.145	0.151	0.148

Species

Sus scrofa

Sensitivity

9.38 ng/mL

Limit Of Detection

450 nm

UniProt ID	P79263
Antigen	inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)

Applications

ELISA

Research Area

ITIH4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507168	CSB-E13425p	96T	$790	Add to Cart
BHE10507168	CSB-E13425p	5x96T	$3792	Add to Cart
BHE10507168	CSB-E13425p	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.