Pig Keratinocyte Growth Factor,KGF ELISA Kit

Mfr.No.

CSB-E06800p

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 7 (keratinocyte growth factor), HBGF-7, KGF, fibroblast growth factor 7, heparin-binding growth factor 7, keratinocyte growth factor

Recovery

The recovery of human PTK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	91	84-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PTK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	92
	Range %	87-101
1:4	Average %	98
	Range %	94-104
1:8	Average %	98
	Range %	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.274	2.265	2.270	2.161
400	1.779	1.812	1.796	1.687
200	1.134	1.158	1.146	1.037
100	0.660	0.712	0.686	0.577
50	0.385	0.410	0.398	0.289
25	0.233	0.235	0.234	0.125
12.5	0.174	0.187	0.181	0.072
0	0.107	0.110	0.109	?

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9N198

Applications	ELISA
Research Area	FGF7

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507157	CSB-E06800p	96T	$658	Add to Cart
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BHE10507157	CSB-E06800p	10x96T	$6251	Add to Cart

Pig Keratinocyte Growth Factor,KGF ELISA Kit

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