Pig Japanese Encephalitis Antibody(IgG)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027220PI

Sensitivity

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Detection Range

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Reactivity

Pig

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

Japanese Encephalitis Antibody(IgG)

Recovery

The recovery of human C9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	97	87-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:2000	Average %	96
	Range %	90-104
1:4000	Average %	91
	Range %	87-98
1:8000	Average %	95
	Range %	90-110
1:16000	Average %	96
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Detection of Japanese encephalitis viral antibodies from swine Sonuwara Begum.et al,The Pharma Innovation Journal ,Oct

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