Pig interleukin 17,IL-17 ELISA Kit

Pig interleukin 17,IL-17 ELISA Kit

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Mfr.No.

CSB-E08057p

Alternative Names

interleukin 17A, CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, cytotoxic T-lymphocyte-associated serine esterase 8, interleukin 17 (cytotoxic T-ly

Reactivity

Pig

Detection Range

20 pg/mL- 800 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-92
1:200	Average %	97
	Range %	91-105
1:400	Average %	85
	Range %	82-89
1:800	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.751	2.752	2.752	2.559
250	2.327	2.377	2.352	2.159
125	1.700	1.675	1.688	1.495
62.5	0.993	0.968	0.981	0.788
31.2	0.637	0.625	0.631	0.438
15.6	0.401	0.416	0.409	0.216
7.8	0.299	0.289	0.294	0.101
0	0.188	0.197	0.193	?

Species

Sus scrofa

Sensitivity

10 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q60I29
Antigen	interleukin 17A

Applications

ELISA

Research Area

IL17A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507136	CSB-E08057p	96T	$496	Add to Cart
BHE10507136	CSB-E08057p	5x96T	$2380.80	Add to Cart
BHE10507136	CSB-E08057p	10x96T	$4712	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.