Pig insulin-like growth factors 2,IGF-2 ELISA Kit

Pig insulin-like growth factors 2,IGF-2 ELISA Kit

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Mfr.No.

CSB-E06830p

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor 2 (somatomedin A), C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018, OTTHUMP00000011157, insulin-like growth factor 2, insulin-like growth factor II, insulin-like growth factor type 2, putative insulin-like

Recovery

The recovery of human REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	90	85-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	99
	Range %	95-107
1:4	Average %	104
	Range %	100-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.519	2.352	2.436	2.296
100	1.923	1.856	1.890	1.750
50	1.301	1.254	1.278	1.138
25	0.719	0.721	0.720	0.580
12.5	0.415	0.387	0.401	0.261
6.25	0.324	0.307	0.316	0.176
3.12	0.215	0.229	0.222	0.082
0	0.142	0.137	0.140

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P23695

Applications	ELISA
Research Area	IGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507122	CSB-E06830p	96T	$615	Add to Cart
BHE10507122	CSB-E06830p	5x96T	$2952	Add to Cart
BHE10507122	CSB-E06830p	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.