Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E06829p

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

Recovery

The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	99-110
EDTA plasma (n=4)	92	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	89
	Range %	82-94
1:10	Average %	96
	Range %	91-105
1:20	Average %	99
	Range %	95-108
1:40	Average %	94
	Range %	86-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P16545

Applications	ELISA
Research Area	IGF1

Storage	Store at 2-8°C. Please refer to protocol.

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