Pig inducible nitric oxide synthase(iNOS)ELISA Kit

Pig inducible nitric oxide synthase(iNOS)ELISA Kit

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Mfr.No.

CSB-E17489p

Alternative Names

inducible nitric oxide synthase(iNOS), HEP-NOS, INOS, NOS, NOS2A, NOS, type II, nitric oxide synthase 2A, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage

Reactivity

Pig

Detection Range

0.05 U/mL-40 U/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human LMNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-87
EDTA plasma (n=4)	94	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LMNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-91
1:2	Average %	103
	Range %	100-106
1:4	Average %	85
	Range %	82-89
1:8	Average %	96
	Range %	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.434	2.468	2.451	2.302
500	1.661	1.698	1.680	1.531
250	1.094	1.072	1.083	0.934
125	0.656	0.627	0.642	0.493
62.5	0.426	0.442	0.434	0.285
31.2	0.309	0.318	0.314	0.165
15.6	0.258	0.248	0.253	0.104
0	0.150	0.148	0.149

Species

Sus scrofa

Sensitivity

0.05 U/mL

Limit Of Detection

450 nm

UniProt ID	P29477
Antigen	inducible nitric oxide synthase(iNOS)

Applications

ELISA

Research Area

iNOS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507116	CSB-E17489p	96T	$695	Add to Cart
BHE10507116	CSB-E17489p	5x96T	$3336	Add to Cart
BHE10507116	CSB-E17489p	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.