Pig Immunoglobulin A,IgA ELISA Kit

Mfr.No.

CSB-E13234p

Sensitivity

0.146 ug/mL

Detection Range

0.146 ug/mL-37.5 ug/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Immunoglobulin A,IgA

Recovery

The recovery of human DAPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	97	92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DAPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	87
	Range %	82-94
1:4	Average %	93
	Range %	87-98
1:8	Average %	104
	Range %	100-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.609	2.534	2.572	2.487
1000	1.624	1.701	1.663	1.578
500	0.912	0.879	0.896	0.811
250	0.531	0.512	0.522	0.437
125	0.274	0.267	0.271	0.186
62.5	0.246	0.235	0.241	0.156
31.25	0.129	0.133	0.131	0.046
0	0.083	0.086	0.085	?

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012 Jun;35(2):225-30 Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets Fengrui Zhang et al,PLoS One,2013 Oral administration of N-carbamylglutamate might improve growth performance and intestinal function of suckling piglets Xiangfang Zeng.et al,Livestock Science,181: 242-248 Effects of dietary supplementation with cupreous N-carbamylglutamate (NCG) chelate and copper sulfate on growth performance, serum biochemical profile and immune response, tissue mineral levels and fecal excretion of mineral in weaning piglets Peng Liao.et al,Food and Agricultural Immunology ,Jul 1 Effect of dietary copper source (inorganic vs. chelated) on immune response, mineral status, and fecal mineral excretion in nursery piglets Peng Liao.et al,Food and Agricultural Immunology ,Dec 17 Effects of dietary supplementation with essential oils and organic acids on the growth performance, immune system, faecal volatile fatty acids and microflora community in weaned piglets Yang CM, et al,Journal of Animal Science,November 2 Increasing selenium supply for heat-stressed or actively cooled sows improves piglet preweaning survival, colostrum and milk composition, as well as maternal selenium, antioxidant status and immunoglobulin transfer JunChen, et al,Journal of trace elements in medicine and biology,November 29 Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,December 24

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10507112	CSB-E13234p	96T	$790	Add to Cart
BHE10507112	CSB-E13234p	5x96T	$3792	Add to Cart
BHE10507112	CSB-E13234p	10x96T	$7505	Add to Cart

Pig Immunoglobulin A,IgA ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Pig Immunoglobulin A,IgA ELISA Kit

Rate it now..!!