Pig HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

Mfr.No.

ELK9014

Sensitivity

1.27 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Pig

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70

Recovery

Matrices listed below were spiked with certain level of recombinant HSP-70/HSPA9 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70/HSPA9 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-107%	98%
EDTA plasma(n=5)	79-91%	85%
Heparin plasma(n=5)	81-97%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70/HSPA9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-103%	83-94%	92-105%	89-97%
EDTA plasma(n=5)	87-102%	85-94%	87-101%	82-96%
Heparin plasma(n=5)	95-102%	87-98%	81-97%	85-92%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	2.354	2.246
100.00	1.687	1.579
50.00	1.227	1.119
25.00	0.958	0.850
12.50	0.621	0.513
6.25	0.402	0.294
3.13	0.248	0.140
0.00	0.108	0.000

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P48721

Applications	ELISA
Research Area	Tumor immunity;Infection immunity;Developmental science;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207690	ELK9014	48T	$412		Add to Cart
BHE15207690	ELK9014	96T	$588		Add to Cart

Pig HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

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