Pig Haptoglobin,Hpt/HP ELISA Kit

Pig Haptoglobin,Hpt/HP ELISA Kit

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Mfr.No.

CSB-E13424p

Sensitivity

0.39 ug/mL

Detection Range

1.56 ug/mL-100 ug/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

haptoglobin, BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide

Recovery

The recovery of human CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-94
EDTA plasma (n=4)	97	95-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-103
1:2	Average %	93
	Range %	85-98
1:4	Average %	99
	Range %	95-106
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2800	1.830	1.952	1.891	1.791
1400	1.008	1.076	1.042	0.942
700	0.636	0.604	0.620	0.520
350	0.351	0.369	0.360	0.260
175	0.218	0.224	0.221	0.121
87.5	0.161	0.159	0.160	0.060
43.75	0.123	0.129	0.126	0.026
0	0.103	0.097	0.100	?

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8SPS7

Applications	ELISA
Research Area	HP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507099	CSB-E13424p	96T	$790	Add to Cart
BHE10507099	CSB-E13424p	5x96T	$3792	Add to Cart
BHE10507099	CSB-E13424p	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.