Pig glial fibrillary acidic protein,GFAP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14950p

Sensitivity

0.05 ng/mL

Detection Range

0.1 ng/mL-20 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

glial fibrillary acidic protein,GFAP

Recovery

The recovery of rat MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	97	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	103
	Range %	98-105
1:4	Average %	93
	Range %	88-97
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	F1RR02

Applications	ELISA
Research Area	GFAP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Can Recognition of Spinal Ischemia Be Improved-Application of Motor-Evoked Potentials, SerumMarkers, and Breath Gas Analysis in an Acutely Instrumented Pig Model Anja Puschel.et al,ANN VASC SURG,April Can recognition of spinal ischemia be improved-Application of motor-evoked potentials, serumMarkers and breath gas analysis in an acutely instrumented pig model Pschel A.et al,Ann Vasc Surg,Mar 5

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