Pig free tri-iodothyronine (FT3) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQF027510PI

Sensitivity

0.38 pmol/L

Detection Range

2 pmol/L-32 pmol/L

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

free tri-iodothyronine indes(FT3)

Recovery

The recovery of human COL ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	93	85-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human COL ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	86
	Range %	80-104
1:400	Average %	97
	Range %	91-105
1:800	Average %	100
	Range %	92-113
1:1600	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	FT3

Storage	Store at 2-8°C. Please refer to protocol.

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