Pig FGFR2(Fibroblast Growth Factor Receptor 2) ELISA Kit

Mfr.No.

ELK8484

Sensitivity

12.2 pg/mL

Detection Range

31.25-2000 pg/mL

Reactivity

Pig

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD332; BEK; BFR1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor; Craniofacial Dysostosis 1; Crouzon Syndrome

Recovery

Matrices listed below were spiked with certain level of recombinant FGFR2 and the recovery rates were calculated by comparing the measured value to the expected amount of FGFR2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	81-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-100%	84-99%	85-97%	88-102%
EDTA plasma(n=5)	92-102%	81-93%	86-98%	91-103%
Heparin plasma(n=5)	85-97%	87-96%	85-96%	85-95%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.997	1.906
1000.00	1.527	1.436
500.00	1.128	1.037
250.00	0.791	0.700
125.00	0.509	0.418
62.50	0.305	0.214
31.25	0.230	0.139
0.00	0.091	0.000

Limit Of Detection

2000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;CD & Adhesion molecule;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207704	ELK8484	48T	$320		Add to Cart
BHE15207704	ELK8484	96T	$458		Add to Cart

Pig FGFR2(Fibroblast Growth Factor Receptor 2) ELISA Kit

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