Pig Ferritin heavy chain(FTH1) ELISA kit

Pig Ferritin heavy chain(FTH1) ELISA kit

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Mfr.No.

CSB-EL009030PI

Sensitivity

1.175 ng/ml

Detection Range

4.7 ng/ml- 300 ng/ml

Reactivity

Pig

Assay Duration

1-5h

Alternative Names

ferritin, heavy polypeptide 1, FTH1 ELISA Kit; FTHFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) (Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed) ELISA Kit

Recovery

The recovery of human Tie-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	91	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Tie-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	83-92
1:10	Average %	106
	Range %	101-110
1:20	Average %	99
	Range %	95-103
1:40	Average %	98
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.531	2.501	2.516	2.402
30	2.018	2.121	2.070	1.956
15	1.505	1.568	1.537	1.423
7.5	1.040	1.068	1.054	0.940
3.75	0.657	0.687	0.672	0.558
1.88	0.421	0.432	0.427	0.313
0.94	0.271	0.290	0.281	0.167
0	0.112	0.115	0.114

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P19130

Applications	ELISA
Research Area	FTH1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507069	CSB-EL009030PI	96T	$595	Add to Cart
BHE10507069	CSB-EL009030PI	5x96T	$2856	Add to Cart
BHE10507069	CSB-EL009030PI	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.