Pig Epithelial growth factor,EGF ELISA Kit

Mfr.No.

CSB-E06788p

Alternative Names

Epithelial growth factor,EGF

Reactivity

Pig

Detection Range

250 pg/mL-10000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	86
	Range %	81-97
1:10	Average %	89
	Range %	85-100
1:20	Average %	95
	Range %	90-100
1:40	Average %	93
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

125 pg/mL

Limit Of Detection

450 nm

UniProt ID	F1S137
Antigen	Epithelial growth factor,EGF

Applications

ELISA

Research Area

EGF

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10507061	CSB-E06788p	10x96T	$4940	Add to Cart