Pig Corticoliberin(CRH) ELISA kit

Mfr.No.

CSB-EL005963PI

Sensitivity

100 pg/mL

Detection Range

100 pg/mL-1600 pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

corticotropin releasing hormone, CRF, corticotropin-releasing factor

Recovery

The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	106	101-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P06296

Applications	ELISA
Research Area	CRH

Storage	Store at 2-8°C. Please refer to protocol.

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