Pig complement fragment 5a (C5a) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027637PI

Sensitivity

2.34 ng/mL

Detection Range

9.375 ng/mL-600 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement fragment 5a (C5a)

Recovery

The recovery of human SEMA7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	103	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-97
1:2	Average %	92
	Range %	82-96
1:4	Average %	94
	Range %	84-98
1:8	Average %	87
	Range %	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	C5a

Storage	Store at 2-8°C. Please refer to protocol.

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