Pig Collagen Type III,Col III ELISA Kit

Pig Collagen Type III,Col III ELISA Kit

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Mfr.No.

CSB-E06776p

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III, Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III

Recovery

The recovery of mouse apelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-109
EDTA plasma (n=4)	96	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse apelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.387	2.272	2.330	2.216
1000	1.721	1.832	1.777	1.663
500	1.273	1.239	1.256	1.142
250	0.709	0.743	0.726	0.612
125	0.412	0.441	0.427	0.313
62.5	0.264	0.278	0.271	0.157
31.25	0.205	0.214	0.210	0.096
0	0.116	0.112	0.114

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col III

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507033	CSB-E06776p	96T	$856	Add to Cart
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BHE10507033	CSB-E06776p	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.