Pig collagen type I (COLI) ELISA kit

Pig collagen type I (COLI) ELISA kit

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Mfr.No.

CSB-EQ027853PI

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen type I (COLI), collagen type 1 (COL1),_x000D_ collagen type I (COLI), collagen type I (COLI), collagen type 1 (COL1),_x000D_ collagen type I (COLI)

Recovery

The recovery of mouse C4a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	87-105
EDTA plasma (n=4)	96	82-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	87-105
1:2000	Average %	90
	Range %	84-100
1:4000	Average %	95
	Range %	82-103
1:8000	Average %	96
	Range %	84-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.786	2.687	2.737	2.599
1250	2.136	2.042	2.089	1.951
625	1.253	1.265	1.259	1.121
312	0.784	0.752	0.768	0.630
156	0.543	0.521	0.532	0.394
78	0.364	0.342	0.353	0.215
39	0.242	0.239	0.241	0.103
0	0.136	0.140	0.138

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	COLI

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507034	CSB-EQ027853PI	96T	$790	Add to Cart
BHE10507034	CSB-EQ027853PI	5x96T	$3792	Add to Cart
BHE10507034	CSB-EQ027853PI	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.