Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL002655PI

Sensitivity

2.35 ng/mL

Detection Range

9.375 ng/mL-600 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

brain-derived neurotrophic factor, MGC34632, neurotrophin

Recovery

The recovery of human Trx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-97
EDTA plasma (n=4)	97	94-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Trx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-105
1:2	Average %	99
	Range %	95-102
1:4	Average %	96
	Range %	92-100
1:8	Average %	95
	Range %	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P14082

Applications	ELISA
Research Area	BDNF

Storage	Store at 2-8°C. Please refer to protocol.

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