Pig basic fibroblast growth factor,bFGF ELISA Kit

Pig basic fibroblast growth factor,bFGF ELISA Kit

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Mfr.No.

CSB-E12841p

Sensitivity

1.2 pg/mL

Detection Range

4.7 pg/mL-300 pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 2 (basic), BFGF, FGFB, HBGF-2, basic fibroblast growth factor bFGF, fibroblast growth factor 2, heparin-binding growth factor 2, prostatropin

Recovery

The recovery of human MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-108
EDTA plasma (n=4)	94	86-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10000	Average %	90
	Range %	83-103
1:20000	Average %	92
	Range %	89-97
1:40000	Average %	96
	Range %	89-105
1:80000	Average %	95
	Range %	86-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
5	0.155	0.167	0.161
2.5	0.304	0.299	0.302
1.25	0.448	0.459	0.454
0.625	0.730	0.794	0.762
0.312	1.212	1.228	1.220
0.156	1.733	1.701	1.717
0.078	1.993	2.006	2.000
0	2.501	2.529	2.515

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q60487

Applications	ELISA
Research Area	FGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506993	CSB-E12841p	96T	$856	Add to Cart
BHE10506993	CSB-E12841p	5x96T	$4108.80	Add to Cart
BHE10506993	CSB-E12841p	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.