Pig apolipoprotein A1 (Apo-A1) ELISA Kit

Mfr.No.

CSB-E06834p

Alternative Names

apolipoprotein A-I, MGC117399, OTTHUMP00000069346, OTTHUMP00000069347, OTTHUMP00000069348

Reactivity

Pig

Detection Range

80 ug/mL-2000 ug/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human TSG101 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	99-106
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TSG101 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	108
	Range %	103-112
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

50 ug/mL

Limit Of Detection

450 nm

UniProt ID	P18648
Antigen	apoLipoprotein A-I

Applications

ELISA

Research Area

APOA1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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