Pig Angiopoietin 1,ANG-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08457p

Alternative Names

angiopoietin 1, AGP1, AGPT, ANG1

Reactivity

Pig

Detection Range

6 ng/mL-200 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Duration

1-5h

Recovery

The recovery of human VLDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VLDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

6 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q9BDY8
Antigen	Angiopoietin 1

Applications

ELISA

Research Area

ANGPT1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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