Pig adiponectin,ADP ELISA Kit

Pig adiponectin,ADP ELISA Kit

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Mfr.No.

CSB-E06839p

Alternative Names

adiponectin,ADP

Reactivity

Pig

Detection Range

1.875 ug/mL-30 ug/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rabbit PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	98
	Range %	93-104
1:4	Average %	91
	Range %	86-97
1:8	Average %	89
	Range %	87-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.911	3.020	2.966	2.827
250	2.405	2.510	2.458	2.319
125	1.756	1.772	1.764	1.625
62.5	1.106	1.189	1.148	1.009
31.2	0.582	0.613	0.598	0.459
15.6	0.490	0.457	0.474	0.335
7.8	0.300	0.305	0.303	0.164
0	0.134	0.144	0.139

Species

Sus scrofa

Sensitivity

1.875 ug/mL

Limit Of Detection

450 nm

UniProt ID	Q6PP07
Antigen	adiponectin,ADP

Applications

ELISA

Research Area

ADP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig Maleszka A et al,Acta Vet Hung,2014 Mar 22:1-11. Body condition of gilts at the end of gestation affects their mammary development. Farmer C.et al,J Anim Sci.,94(5):1897-905 Branched-chain amino acid ratios modulate lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,Journal of Functional Foods,Jan Low-protein diet improves meat quality of growing and finishing pigs through changing lipid metabolism, fiber characteristics, and free amino acid profile of the muscle Y H Li.et al,Journal of Animal Science,July 10 β-hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,FOOD & FUNCTION,August 16 Body condition of late pregnant gilts affects the expression of selected adipokines and their receptors in mammary fat and backfat tissues Duarte CRA.et al,journal of animal science,Octobert 12

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.