Pig activated protein C(APC) ELISA Kit

Mfr.No.

CSB-E12633p

Sensitivity

20 pg/mL

Detection Range

40 pg/mL-8000 pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

activated protein C(APC)

Recovery

The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	88-96
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	95
	Range %	90-100
1:8000	Average %	88
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	APC

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506966	CSB-E12633p	10x96T	$7505	Add to Cart