Matrices listed below were spiked with certain level of PGE1 and the recovery rates were calculated by comparing the measured value to the expected amount of PGE1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
97
EDTA plasma(n=5)
89-103
97
heparin plasma(n=5)
85-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGE1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
89-105%
89-104%
EDTA plasma(n=5)
88-93%
88-98%
88-96%
heparin plasma(n=5)
86-100%
80-100%
80-95%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
PGE1 ELISA Kit allows for the in vitro quantitative determination of PGE1 concentrations in serum, plasma, tissue homogenates and other biological fluids.