Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of OVA and the recovery rates were calculated by comparing the measured value to the expected amount of OVA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
91
EDTA plasma(n=5)
85-101
94
heparin plasma(n=5)
88-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
85-102%
85-101%
EDTA plasma(n=5)
83-101%
87-100%
82-93%
heparin plasma(n=5)
81-95%
90-100%
82-91%
Sensitivity
< 0.469ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
OVA ELISA Kit allows for the in vitro quantitative determination of OVA concentrations in serum, plasma, tissue homogenates and other biological fluids.