NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit

Mfr.No.

ELK8020

Sensitivity

48.7 ng/mL

Detection Range

156.25-10000 ng/mL

Reactivity

General

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

NAADP

Recovery

Matrices listed below were spiked with certain level of recombinant NAADP and the recovery rates were calculated by comparing the measured value to the expected amount of NAADP in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	94-107%	100%
Heparin plasma(n=5)	87-99%	94%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NAADP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-104%	93-105%	87-103%	91-102%
EDTA plasma(n=5)	79-95%	96-105%	85-96%	90-99%
Heparin plasma(n=5)	79-91%	83-97%	81-103%	89-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10000.00	0.195
5000.00	0.413
2500.00	0.612
1250.00	0.993
625.00	1.297
312.50	1.589
156.25	1.889
0.00	2.219

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

10000 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200411	ELK8020	48T	$265		Add to Cart
BHE15200411	ELK8020	96T	$376		Add to Cart

NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit

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