Mouse Zona Pellucida Sperm-binding Protein 3

Mfr.No.

E2573Mo

Sensitivity

9.13ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Processed zona pellucida sperm-binding protein 3;Sperm receptor;Zona pellucida glycoprotein 3;Zona pellucida protein C;Zona pellucida sperm-binding protein 3;ZP 3;ZP3;Zp-3

Molecular Weight

46304

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2573Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1397.1	42.26	3
Intra-Assay	2	18	563.6	23.56	4.2
Intra-Assay	3	18	328.6	21.79	6.6

Background

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Protocol

These standard curves of E2573Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.561	2.458	2.425
	2.355
1200ng/L	1.494	1.441	1.408
	1.388
600ng/L	0.768	0.849	0.817
	0.93
300ng/L	0.51	0.501	0.469
	0.493
150ng/L	0.222	0.249	0.216
	0.275
0ng/L	0.028	0.033	0
	0.037

Gene ID	22788
UniProt ID	P10761

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110384	E2573Mo	48T	$320		Add to Cart
BHE12110384	E2573Mo	96T	$458		Add to Cart

Mouse Zona Pellucida Sperm-binding Protein 3 - ZP3 ELISA Kit

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