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Home / ELISA Kit

Mouse Zinc finger transcription factor Trps1 (TRPS1) ELISA Kit

SKU : BHE13705215

TRPV1 is a nonselective cation channel that may be activated by a wide variety of exogenous and endogenous physical and chemical stimuli. The best-known activators of TRPV1 are heat greater than 43°C and capsaicin, the pungent compound in hot chili peppers.
The activation of TRPV1 leads to painful, burning sensation. Its endogenous activators include: low pH, the endocannabinoid anandamide, N-arachidonoyl-dopamine. TRPV1 receptors are found mainly in the nociceptive neurons of the peripheral nervous system, but they have also been described in many other tissues, including the central nervous system. TRPV1 is involved in the transmission and modulation of pain, as well as the integration of diverse painful stimuli.

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Mfr.No.	KTE70053
Description	This Mouse Zinc finger transcription factor Trps1 (TRPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPS1.
Alternative Names	TRPS1; GC79; LGCR; MGC134928; zinc finger transcription factor TRPS1
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Zinc finger transcription factor Trps1 microplate Mouse Zinc finger transcription factor Trps1 standard Mouse Zinc finger transcription factor Trps1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Zinc finger transcription factor Trps1 (TRPS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPS1. No significant cross-reactivity or interference between Mouse TRPS1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRPV1 is a nonselective cation channel that may be activated by a wide variety of exogenous and endogenous physical and chemical stimuli. The best-known activators of TRPV1 are heat greater than 43°C and capsaicin, the pungent compound in hot chili peppers. The activation of TRPV1 leads to painful, burning sensation. Its endogenous activators include: low pH, the endocannabinoid anandamide, N-arachidonoyl-dopamine. TRPV1 receptors are found mainly in the nociceptive neurons of the peripheral nervous system, but they have also been described in many other tissues, including the central nervous system. TRPV1 is involved in the transmission and modulation of pain, as well as the integration of diverse painful stimuli.
Notes	This Mouse Zinc finger transcription factor Trps1 (TRPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPS1 in samples. An antibody specific for TRPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	TRPS1
Tag	TRPS1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Zinc finger transcription factor Trps1 (TRPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPS1 in samples. An antibody specific for TRPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705215	KTE70053	48 T	$339		Add to Cart
BHE13705215	KTE70053	96 T	$589		Add to Cart
BHE13705215	KTE70053	96 Tx5	$2629		Add to Cart
BHE13705215	KTE70053	96 Tx50	$24569		Add to Cart

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