Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®

Mfr.No.

EK2010

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.030	0.076	0.123	0.221	0.403	0.754	1.313	2.035

Description

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Chil3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Chitinase-like protein 3; Beta-N-acetylhexosaminidase Ym1; Chitinase-3-like protein 3; ECF-L; Eosinophil chemotactic cytokine; Secreted protein Ym1; Chil3; Chi3l3; Ym1

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-Y398

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CHI3L3

Subcellular Localization

Nucleus envelope. Secreted. Rough endoplasmic reticulum lumen. Cytoplasm. Cytoplasmic granule.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	327	1000	4032	308	1073	3654
Standard deviation	13.73	67	221.76	18.78	83.69	204.62
CV (%)	4.2%	6.7%	5.5%	6.1%	7.8%	5.6%

Protein Function

Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.

Background

Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	327	332	292	319	317	15.43	4.8%
Sample 2	1000	1134	1146	1166	1111	65.38	5.8%
Sample 3	4032	3785	4495	4279	4147	265.89	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12655
Gene Name	Chil3
UniProt ID	O35744
Protein Name	Chitinase-like protein 3
Tissue Specificity	Expressed in peritoneal cavity macrophages and in peritoneal and bone marrow-derived neutrophils. Abundantly expressed in bone marrow, with moderate levels detected in gastric antrum, spleen and in alveolar macrophages in lung. Not detected in brain, heart, liver, kidney, stomach, intestine, skeletal muscle, ovary, testis, thymus and lymph nodes (at protein level). Detected at low levels in bone marrow, spleen, thymus and lung. Barely detectable in intestine, kidney and coecum.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001546	EK2010	96 wells/kit	$750		Add to Cart

Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®

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