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Home / ELISA Kit

Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit

SKU : BHE13705165

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification.
The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

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Mfr.No.	KTE70003
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit employs a two-site sandwich ELISA to quantitate XDH.
Alternative Names	XDH; XO; XOR; xanthene dehydrogenase; xanthine oxidase; xanthine oxidoreductase
Detection Method	Colorimetric
Kit Components	Mouse Xanthine dehydrogenase/oxidase microplate Mouse Xanthine dehydrogenase/oxidase standard Mouse Xanthine dehydrogenase/oxidase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse XDH. No significant cross-reactivity or interference between Mouse XDH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Notes	This Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit employs a two-site sandwich ELISA to quantitate XDH in samples. An antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22436

Applications	ELISA
Applications Notes	This Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit employs a two-site sandwich ELISA to quantitate XDH in samples. An antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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