Mouse Wnt5a(Protein Wnt-5a) ELISA Kit

Mfr.No.

EM0497

Alternative Names

Wnt5a ELISA Kit, WNT5A ELISA Kit, Protein Wnt-5a ELISA Kit, wingless-type MMTV integRation site family ELISA Kit, member 5A ELISA Kit, WNT-5A protein ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Wnt5a and the recovery rates were calculated by comparing the measured value to the expected amount of Wnt5a in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-102	94
EDTA plasma(n=5)	87-102	91
heparin plasma(n=5)	86-96	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wnt5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-105%	89-103%	88-105%
EDTA plasma(n=5)	82-89%	84-98%	82-99%
heparin plasma(n=5)	83-95%	85-100%	86-98%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Wnt5a/WNT5A/Protein Wnt-5a/wingless-type MMTV integration site family, member 5A/WNT-5A protein

Notes

For Research Use Only

UniProt ID	P22725
Antigen	WNT5A

Applications	ELISA
Applications Notes	Wnt5a ELISA Kit allows for the in vitro quantitative determination of Wnt5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804122	EM0497	96T	$400		Add to Cart

Mouse Wnt5a (Protein Wnt-5a) ELISA Kit

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