Mouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Mfr.No.

EK1523

Sensitivity

<10 pg/ml

Detection Range

23.4 pg/ml - 1500 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	23.4	46.9	93.8	187.5	375	750	1500
O.D.	0.052	0.136	0.232	0.343	0.715	1.119	1.682	2.066

Description

Mouse WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Wif1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Wnt inhibitory factor 1;WIF-1;Wif1;

Molecular Weight

41590 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G29-W379

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse WIF1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	40	298	975	37	309	914
Standard deviation	1.92	22.94	39	1.88	28.73	43.87
CV (%)	4.8%	7.7%	4%	4.8%	9.3%	4.8%

Protein Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Background

Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	40	40	45	38	40	2.58	6.4%
Sample 2	298	281	269	282	282	10.3	3.6%
Sample 3	975	1067	1053	1054	1037	36.36	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24117
Gene Name	Wif1
UniProt ID	Q9WUA1
Protein Name	Wnt inhibitory factor 1
Tissue Specificity	Expression highest in heart and lung. Lower in brain and eye.

Applications	ELISA
Research Area	Secreted,Signaling Pathways,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001109	EK1523	96 wells/kit	$499		Add to Cart

Mouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Rate it now..!!