Mouse Vinculin - VCL ELISA Kit

Mfr.No.

E2563Mo

Sensitivity

9.63ng/L

Detection Range

20-3500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

9430097D22;AA571387;AI462105;AW545629;Metavinculin;VCL;Vinculin

Molecular Weight

116717

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2563Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	797.43	38.98	4.89
Intra-Assay	2	18	1893.59	45.16	2.38
Intra-Assay	3	18	726.3	41.02	5.65

Background

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Protocol

These standard curves of E2563Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.717	1.824	1.795
	1.932
1000ng/L	0.886	0.895	0.865
	0.903
500ng/L	0.585	0.548	0.519
	0.511
250ng/L	0.179	0.197	0.168
	0.215
125ng/L	0.141	0.108	0.079
	0.075
0ng/L	0.032	0.029	0
	0.027

Gene ID	22330
UniProt ID	Q64727

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110374	E2563Mo	48T	$320		Add to Cart
BHE12110374	E2563Mo	96T	$458		Add to Cart

Mouse Vinculin - VCL ELISA Kit

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