Mouse Vinculin(VCL) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL025812MO

Sensitivity

7.03 pg/mL

Detection Range

28.1 pg/mL-1800 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

vinculin, RP11-178G16.3, CMD1W, MVCL, metavinculin

Recovery

The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	94
	Range %	88-102
1:8	Average %	96
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q64727

Applications	ELISA
Research Area	VCL

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10506924	CSB-EL025812MO	96T	$595	Add to Cart
BHE10506924	CSB-EL025812MO	5x96T	$2856	Add to Cart
BHE10506924	CSB-EL025812MO	10x96T	$5652.50	Add to Cart