Mouse Vimentin - VIM ELISA Kit

Mfr.No.

E2669Mo

Sensitivity

0.056ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

VIM;Vimentin

Molecular Weight

53688

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2669Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23	0.86	3.7
Intra-Assay	2	18	5.6	0.31	5.5
Intra-Assay	3	18	13	0.63	4.8

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Protocol

These standard curves of E2669Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.772	1.886	1.842
	2
12ng/ml	1.081	1.029	0.985
	0.978
6ng/ml	0.466	0.558	0.513
	0.649
3ng/ml	0.274	0.268	0.224
	0.262
1.5ng/ml	0.133	0.11	0.066
	0.087
0ng/ml	0.062	0.044	0
	0.027

Gene ID	22352
UniProt ID	P20152

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115498	E2669Mo	48T	$320		Add to Cart
BHE12115498	E2669Mo	96T	$458		Add to Cart

Mouse Vimentin - VIM ELISA Kit

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