Matrices listed below were spiked with certain level of VCAN and the recovery rates were calculated by comparing the measured value to the expected amount of VCAN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
95
EDTA plasma(n=5)
89-104
96
heparin plasma(n=5)
89-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCAN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
85-101%
95-103%
EDTA plasma(n=5)
82-97%
84-100%
86-101%
heparin plasma(n=5)
80-90%
90-97%
84-93%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q62059
Applications
ELISA
Applications Notes
VCAN ELISA Kit allows for the in vitro quantitative determination of VCAN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse VCAN(Versican core protein) ELISA Kit