Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

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Mfr.No.

CSB-E04756m

Sensitivity

0.857 pg/mL

Detection Range

3.906 pg/mL-250 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

vascular endothelial growth factor A, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor

Recovery

The recovery of human BAT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-96
EDTA plasma (n=4)	87	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BAT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	96
	Range %	91-101
1:4	Average %	90
	Range %	87-95
1:8	Average %	98
	Range %	96-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.086	2.210	2.148	2.055
2000	1.517	1.587	1.552	1.459
1000	0.947	0.932	0.940	0.847
500	0.581	0.599	0.590	0.497
250	0.380	0.390	0.385	0.292
125	0.254	0.246	0.250	0.157
62.5	0.173	0.177	0.175	0.082
0	0.093	0.092	0.093	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q00731

Applications	ELISA
Research Area	VEGFA

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.