Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

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Mfr.No.

CSB-E04764m

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

kinase insert domain receptor (a type III receptor tyrosine kinase), CD309, FLK1, VEGFR, VEGFR2, fetal liver kinase-1, kinase insert domain receptor, protein-tyrosine kinase receptor Flk-1, soluble VEGFR2, tyrosine kinase growth factor receptor, vascular endothelial growt

Recovery

The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	101
	Range %	95-107
1:200	Average %	94
	Range %	90-100
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.093	0.101	0.097
20	0.209	0.216	0.213
10	0.422	0.441	0.432
5	0.739	0.715	0.727
2.5	1.168	1.124	1.146
1.25	1.602	1.709	1.656
0.625	1.779	1.879	1.829
0	2.170	2.297	2.234

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	P35918

Applications	ELISA
Research Area	VEGFR-2, Flk-1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.