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Home / ELISA Kit

Mouse V-type Proton Atpase Subunit E 1 - ATP6V0E1 ELISA Kit

SKU : BHE12110377

Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E2566Mo

Sensitivity

9.19ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Atp;Atp6k;Atp6v0e1;M9.2;Vacuolar proton pump subunit e 1;V-ATPase subunit e 1;V-type proton ATPase subunit e 1

Molecular Weight

9340

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2566Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	959.32	39.38	4.11
Intra-Assay	2	18	1378.69	48.81	3.54
Intra-Assay	3	18	420.59	18.96	4.51

Background

Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Protocol

These standard curves of E2566Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.141	2.184	2.143
	2.228
1200ng/L	1.327	1.35	1.308
	1.373
600ng/L	0.759	0.69	0.649
	0.622
300ng/L	0.444	0.401	0.359
	0.357
150ng/L	0.142	0.176	0.134
	0.209
0ng/L	0.027	0.042	0
	0.056

Gene ID	11974
UniProt ID	Q9CQD8

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110377	E2566Mo	48T	$320		Add to Cart
BHE12110377	E2566Mo	96T	$458		Add to Cart

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