Matrices listed below were spiked with certain level of Ucn and the recovery rates were calculated by comparing the measured value to the expected amount of Ucn in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
88-101
94
heparin plasma(n=5)
88-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ucn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Ucn ELISA Kit allows for the in vitro quantitative determination of Ucn concentrations in serum, plasma, tissue homogenates and other biological fluids.