Mouse Ubiquitin-like protein ISG15(ISG15) ELISA kit

Mouse Ubiquitin-like protein ISG15(ISG15) ELISA kit

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Mfr.No.

CSB-EL011843MO

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ISG15 ubiquitin-like modifier, G1P2, IFI15, UCRP, interferon, alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein, interferon-stimulated protein, 15 kDa, ubiquitin cross-reactive protein

Recovery

The recovery of human LOXL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-98
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LOXL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	88
	Range %	83-92
1:4	Average %	95
	Range %	89-99
1:8	Average %	98
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.438	2.546	2.492	2.364
5	1.528	1.425	1.477	1.349
2.5	0.856	0.902	0.879	0.751
1.25	0.523	0.547	0.535	0.407
0.625	0.318	0.333	0.326	0.198
0.312	0.255	0.264	0.260	0.132
0.156	0.204	0.208	0.206	0.078
0	0.127	0.128	0.128

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q64339

Applications	ELISA
Research Area	ISG15

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506896	CSB-EL011843MO	96T	$695	Add to Cart
BHE10506896	CSB-EL011843MO	5x96T	$3336	Add to Cart
BHE10506896	CSB-EL011843MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.