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Home / ELISA Kit

Mouse Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit

SKU : BHE13705283

This protein is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

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Mfr.No.	KTE70122
Description	This Mouse Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF4.
Alternative Names	TNFRSF4; RP5-902P8.3; ACT35; CD134; OX40; TXGP1L; ATC35 antigen; CD134 antigen; OX40 antigen; OX40 cell surface antigen; OX40 homologue; lymphoid activation antigene ACT35; tax-transcriptionally activated glycopro
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Tumor necrosis factor receptor superfamily member 4 microplate Mouse Tumor necrosis factor receptor superfamily member 4 standard Mouse Tumor necrosis factor receptor superfamily member 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF4. No significant cross-reactivity or interference between Mouse TNFRSF4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This protein is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Notes	This Mouse Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF4 in samples. An antibody specific for TNFRSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	22163
Antigen	TNFRSF4
Tag	TNFRSF4
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF4 in samples. An antibody specific for TNFRSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705283	KTE70122	48 T	$339		Add to Cart
BHE13705283	KTE70122	96 T	$589		Add to Cart
BHE13705283	KTE70122	96 Tx5	$2629		Add to Cart
BHE13705283	KTE70122	96 Tx50	$24569		Add to Cart

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