Mouse Tumor necrosis factor receptor superfamily member 18(TNFRSF18) ELISA kit

Mouse Tumor necrosis factor receptor superfamily member 18(TNFRSF18) ELISA kit

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Mfr.No.

CSB-EL023975MO

Sensitivity

19.5 pg/ml

Detection Range

78 pg/ml - 5000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor receptor superfamily, member 18, AITR, GITR, GITR-D, TNF receptor superfamily activation-inducible protein, activation-inducible TNFR family receptor, glucocorticoid-induced TNFR-related protein

Recovery

The recovery of human SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	95	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	82-96
1:2000	Average %	95
	Range %	90-102
1:4000	Average %	98
	Range %	92-105
1:8000	Average %	87
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.772	2.747	2.760	2.624
250	2.349	2.379	2.364	2.228
125	1.712	1.694	1.703	1.567
62.5	1.056	0.993	1.025	0.889
31.2	0.591	0.552	0.572	0.436
15.6	0.372	0.364	0.368	0.232
7.8	0.225	0.217	0.221	0.085
0	0.134	0.138	0.136	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	O35714

Applications	ELISA
Research Area	TNFRSF18

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506868	CSB-EL023975MO	96T	$595	Add to Cart
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BHE10506868	CSB-EL023975MO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.