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Home / ELISA Kit

Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit

SKU : BHE13705287

Tumor necrosis factor receptor superfamily member 17 is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b, and to lead to NF-kappaB and MAPK8/JNK activation.
This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.The patient from whose tumor cells the 4;16 translocation was derived had a chronic intestinal malabsorption syndrome. Histologic and immunohistochemical studies demonstrated a lymphoproliferative syndrome of mature T cells.

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Mfr.No.	KTE70126
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF17.
Alternative Names	TNFRSF17; BCM; BCMA; CD269; B cell maturation antigen; B-cell maturation factor
Detection Method	Colorimetric
Kit Components	Mouse Tumor necrosis factor receptor superfamily member 17 microplate Mouse Tumor necrosis factor receptor superfamily member 17 standard Mouse Tumor necrosis factor receptor superfamily member 17 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF17. No significant cross-reactivity or interference between Mouse TNFRSF17 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor receptor superfamily member 17 is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b, and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.The patient from whose tumor cells the 4;16 translocation was derived had a chronic intestinal malabsorption syndrome. Histologic and immunohistochemical studies demonstrated a lymphoproliferative syndrome of mature T cells.
Notes	This Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF17 in samples. An antibody specific for TNFRSF17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21935

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF17 in samples. An antibody specific for TNFRSF17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705287	KTE70126	48 T	$339		Add to Cart
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