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Home / ELISA Kit

Mouse Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) ELISA Kit

SKU : BHE13705288

Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system.TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was orignially discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

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Mfr.No.	KTE70127
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF13B.
Alternative Names	TNFRSF13B; CD267; CVID; FLJ39942; MGC133214; MGC39952; TACI; TNFRSF14B; transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B
Detection Method	Colorimetric
Kit Components	Mouse Tumor necrosis factor receptor superfamily member 13B microplate Mouse Tumor necrosis factor receptor superfamily member 13B standard Mouse Tumor necrosis factor receptor superfamily member 13B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF13B. No significant cross-reactivity or interference between Mouse TNFRSF13B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system.TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was orignially discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
Notes	This Mouse Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF13B in samples. An antibody specific for TNFRSF13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF13B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57916

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF13B in samples. An antibody specific for TNFRSF13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF13B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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