Mouse Tumor necrosis factor receptor superfamily member 13B(TNFRSF13B) ELISA kit

Mouse Tumor necrosis factor receptor superfamily member 13B(TNFRSF13B) ELISA kit

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Mfr.No.

CSB-EL023971MO

Sensitivity

19.5 pg/ml

Detection Range

78 pg/ml - 5000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor receptor superfamily, member 13B, CD267, CVID, FLJ39942, MGC133214, MGC39952, TACI, TNFRSF14B, transmembrane activator and CAML interactor, tumor necrosis factor receptor 13B

Recovery

The recovery of rat SOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	95	92-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	93
	Range %	88-95
1:400	Average %	104
	Range %	100-107
1:800	Average %	92
	Range %	87-95
1:1600	Average %	97
	Range %	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.774	2.767	2.771	2.638
500	2.226	2.305	2.266	2.133
250	1.737	1.624	1.681	1.548
125	1.108	1.088	1.098	0.965
62.5	0.636	0.628	0.632	0.499
31.2	0.339	0.321	0.330	0.197
15.6	0.231	0.224	0.228	0.095
0	0.130	0.136	0.133	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	Q9ET35

Applications	ELISA
Research Area	TNFRSF13B

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.